Determination of Antioxidant activities of locally produce rice in Northern and Western part of Nigeria, using Kebbi rice and Igbemo rice as case study
DOI:
https://doi.org/10.61978/catalyx.v1i1.238Keywords:
Antioxidant, Kebbi rice, Igbemo rice, DPPH, FRAPAbstract
This research provides the antioxidant activities of locally produced rice in the Northern and Western regions of Nigeria, with a focus on Kebbi rice and Igbemo rice. The study utilizes the Ferric Reducing Antioxidant Power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays to evaluate the antioxidant capacity of these rice varieties. In which the sample was extracted using solvent extraction method after which the antioxidant (AO) activity of the rice samples as ferric-reducing power, is determined using a modified FRAP (ferric reducing/antioxidant power) assay Sodium phosphate, buffer solution, pipettes, test tubes, centrifugal machine, TCA, KFC and reagent such as Fe(iii) solution were used to analyse the ferric reducing antioxidant power (FRAP) of the sample. Also, Methanolic solution, and spectrophotometer to measure absorbance at 516nm is used to determine the free radical scavenging ability of the extract against DPPH (1, 1- diphenyl-2-picryhydrazyl). Results reveal that Kebbi rice exhibits higher FRAP values compared to Igbemo rice, indicating superior antioxidant activity in the FRAP assay. Conversely, Igbemo rice demonstrates higher DPPH values than Kebbi rice, suggesting greater antioxidant potency in the DPPH assay. These findings underscore the variability in antioxidant properties among different rice varieties and highlight the importance of considering multiple assays for a comprehensive assessment of antioxidant activity. Understanding the antioxidant profiles of indigenous rice varieties like Kebbi and Igbemo rice is crucial for promoting their consumption and enhancing food security in Nigeria. Further research is warranted to elucidate the specific antioxidant compounds responsible for the observed differences and explore their potential health benefits.
References
A, P. Y., B, S. T., & Morita, N. (2007). Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities : science direct : journal of Food Chemistry (Vol. 101, Issue ue 4, pp. 1616–1625).
Adom K, & Liu R. H. (2002). Antioxidant activity of grains. J Agric Food Chem, 50, 6182– 6187.
Balasundram, N., Sundram, K., & Samman, S. (2006). Phenolic compounds in plant and agri-industrial byproducts: antioxidant activity, occurrence, and potential uses. Food Chem, 99, 191–203.
Benzie I F, & Szeto Y. (1999). Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. J Agric Food Chem, 47, 633–636.
Birt D. F, Hendrich, S., & Wang, W. (2001). Dietary agents in cancer prevention: flavonoids and isoflavonoids. Pharmacol Therapeut, 90, 157–177.
Bravo, L. (1998). Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev, 56, 317–333.
Chen, P., Kuo, W., Chiang, C., Chiou, H., Y., H., & Chu, S. (2006). Black rice anthocyanins inhibit cancer cells invasion via repressions of MMPs and uPA expression. Chem-Biol Interact.
Choi, Y., S., J. H., & Lee, J. (2007). Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem, 103, 130–138.
Chung M, Walker P, & Hogstrand. (2006). Dietary phenolic antioxidants, caffeic acid and Trolox, protect rainbow trout gill cells from nitric oxide-induced apoptosis. Aquat Toxicol, 80, 321–328.
Collins A, R. (2005). Assays for oxidative stress and antioxidant status: applications to research into the biological effectiveness of polyphenols. Am J Clin Nutr, 81, 261 –267.
Dimitrios, B. (2006). Sources of natural phenolic antioxidants. Trends Food Sci Tech, 17, 505–512.
Frank B, H. (2003). Plant-based foods and prevention of cardiovascular disease: an overview. The American Journal of Clinical Nutrition, 78(3), 544 –551.
Goffman F. D, & Bergman C. J. (2004). Rice kernel phenolic content and its relationship with antiradical efficiency. J Sci Food Agr.
Gyamfi, M. A., & Y.and, M. (1999). Yoko A.(1999). Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. General Pharmacology: The Vascular System, 32(6), 199, 661–667.
Heim K, E., R., T. A., & J, B. D. (2002). Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem, 13, 527–584.
Heim K, ER. , T. A. R., & J, B. D. (2002). Flavonoid antioxidants: chemistry, metabolism and structure activity relationships. J Nutr Biochem, 13, 527–584.
Hindustan Rubber Industries. (2020). India’s Leading rice rubber rolls manufacturer provides quality products for rice mills. https://www.hindustangroup.net/modern-rice-milling-process
Hu, C., Zawistowski, J., Ling, W., & D, K. D. (2003). Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J Agric Food Chem, 51, 5271–5277.
Hudson E, A., A, D. P., T, K., J, S. M. S., & A, G. (2000). Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidem Biomar, 9, 1163–1170.
Hughes, & Okafor. (2015). Review-21-Introduction to agriculture rice. Course hero-homework help, literacture review chapter (2):13-20 from. https://www.coursehero.com/file/p3dsoq4f.
Ibitoye A, A. (2005). Laboratory manual in basic methods in plant and food analysis. A.A.A.C. Official Methods of Analysis.
Iqbal, S., I, B. M., & Anwar, F. (2005). Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chem, 93, 265–272.
Manac, C., Williamson, G., Morand, C., Scalbert, A., & Rémésy, C. (2006). Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies: national library of medicine.
Oki, T., Masuda, M., Furuta, S., Nishiba, Y., Terahara, N., & Suda, I. (2002). Involvement of anthocyanins and other phenolic compounds in radical‐scavenging activity of purple‐fleshed sweet potato cultivars. Journal of Food Science, 67(5), 1752–1756.
Pulido, R., & S.C, L. B. A. F. (2000). Antioxidant Activity of Dietary Polyphenols As determined by a Modified Ferric Reducing/Antioxidant Power Assay. Journal of Agricultural and Food Chemistry, JO(48,8, 3396–3402.
Raajeswari, & P.A. (2021). Food and nutrition: F01FS12 Composition of Rice grain. https://doi.org/.http://epgp.inflibnet.ac.in/epgpdata/uploads/epgp_content/S000444FN/P000546/M011648/ET/1533209357Q-I.pdf.
Reader, & Nwankoro. (2017). Review-21-Introduction to agriculture rice. Course hero-homework help, literature review chapter (2):13-20 from. https://www.coursehero.com/file/p3dsoq4f
Rickford, S. (2022). The process of rice production. https://knowingrice.com/processes-of-rice-production.
Total global rice consumption. (2024). https://www.statista.com/statistics/255977/total-
Zhao, Z., & Moghadasian, M. H. (2008). Chemistry, natural sources, dietary intake and pharmacokinetic properties of ferulic acid: A review: national library of medicine.
Zhou, J.-M., & Ibrahim, R. K. (2004). Tricin—a potential multifunctional nutraceutical. Phytochemistry Reviews, 9, 413–424.
Zhou, T., Wang, Y., Chen, J.-Q., Araki, H., Jing, Z., Jiang, K., Shen, J., & Tian, D. (2004). Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genes. Molecular Genetics and Genomics, 271, 402–415.
